GLP-ARBRE - Good Laboratory Practices in Molecular Biophysics: an ARBRE Symposium

9:00 - 10:00 (1h)

Onsite registration

Welcome and presentation of ARBRE

10:00 - 11:00 (1h)

Sample quality control & standards

Amphitheatre Marie Curie

Chair - Josef Houser

› Measuring the cellular copy number and antibody affinity of an oncoprotein in a native lipid-bilayer environment - Lena Bauernhofer, Biophysics, Institute of Molecular Biosciences (IMB), NAWI Graz, University of Graz, BioTechMed-Graz, Field of Excellence BioHealth, University of Graz 10:00-10:20 (20min)

› Analytical Ultracentrifugation Sedimentation Velocity for the Characterization of Recombinant Adeno-Associated Virus Vectors - Caroline Mas, Univ. Grenoble Alpes, CNRS, CEA, EMBL, ISBG, F-38000 Grenoble 10:20-10:30 (10min)

› Molecular characterization of lipid metabolism by mass spectrometry and multimodal imaging - Sophie Ayciriex, University of Lyon, University Claude Bernard 1 of Lyon, Institute of Analytical Sciences, CNRS, UMR 5280 10:30-10:40 (10min)

› Benchmarking in biophysics: Insight from the ARBRE society - Josef Houser, Masaryk University [Brno] 10:40-10:50 (10min)

11:00 - 11:15 (15min)

Coffee break

Hall

11:15 - 12:25 (1h10)

Macromolecular Interactions & tools

Amphitheatre Marie Curie

Chair - Paloma Fernandez-Varela

› Engineering fluorescent proteins by rational design - Antoine Royant, Institut de Biologie Structurale, European Synchrotron Radiation Facility (ESRF) 11:15-11:35 (20min)

› Biophysical tools to investigate the structural and dynamic properties of the KH domains of FMRP - Carlo Travaglini Allocatelli, Dip. Scienze Biochimiche, Università degli Studi di Roma "La Sapienza" = Sapienza University [Rome] 11:35-11:55 (20min)

› The CE-ICP-MS coupling, a powerful tool for describing protein/metal interactions - Agnès Hagège, Institut des Sciences Analytiques 11:55-12:05 (10min)

› Conformational space exploration of cryo-EM structures by variability refinement - Vincent Chaptal, Microbiologie moléculaire et biochimie structurale (MMSB) 12:05-12:15 (10min)

12:25 - 13:35 (1h10)

Lunch

Hall

12:25 - 13:35 (1h10)

Poster Session

Hall

13:35 - 14:25 (50min)

Technology alert

Amphitheatre Marie Curie

Chair - Maggy Hologne

› Working smart with FIDA: rapid characterization of proteins and particles directly in crude matrix - Albasini Marion, Fidabio 13:35-13:45 (10min)

› Characterization of Therapeutic Antibodies using Digital SPR - Dina Niculaes, Nicoya Lifesciences Inc. 13:45-13:55 (10min)

› Dynamic Biosensors – How to characterize the mode of action and avidity effects of antibodies using switchSENSE® and RT-IC - Amandine Gontier, Dynamic Biosensors GmbH 13:55-14:05 (10min)

› Pushing The Boundaries in Biomolecular Interaction Analysis with the Creoptix WAVEsystem - Aymeric AUDFRAY, Malvern Panalytical 14:05-14:15 (10min)

14:25 - 15:25 (1h)

Lipids, vesicle & membrane proteins

Amphitheatre Marie Curie

Chair - Patrick England

› Sensing peptide-membrane interactions using quantitative fluorescence microscopy - Valeria Vetri, Università degli studi di Palermo - University of Palermo 14:25-14:45 (20min)

› Time-resolved structural transitions of the multidrug transporter BmrA using rapid kinetics and time-resolved cryoEM' - Loïck Moissonnier, MOISSONNIER 14:45-14:55 (10min)

› BIOPHYSICAL TECHNIQUES IN CANCER-DERIVED EXTRACELLULAR VESICLES: AN FT-IR SPECTROSCOPY APPROACH - Sabrina Romanò, Università cattolica del Sacro Cuore = Catholic University of the Sacred Heart [Roma] 14:55-15:05 (10min)

› IMPACT OF MEMBRANE LIPID POLYUNSATURATION ON DOPAMINE D2 RECEPTOR LIGAND BINDING AND SIGNALING - Isabel Alves, CBMN, UMR 5248 15:05-15:15 (10min)

15:25 - 15:35 (10min)

Refreshment

Hall

15:35 - 17:15 (1h40)

ARBRE General Assembly

Amphitheatre Marie Curie

Chair - Stefan H. Knauer

15:35 - 17:15 (1h40)

Poster Session

Hall

17:15 - 17:30 (15min)

Closing remarks

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